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I am trying to download the transcriptomic data for PRJNA121473. From the error message I do not understand what I can change to fix my issue.
Command used and terminal output
Launching `https://github.com/nf-core/fetchngs` [ecstatic_waddington] DSL2 - revision: 8ec2d934f9 [master]WARN: Invalid config manifest attribute `id`WARN: Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value`------------------------------------------------------ ,--./,-. ___ __ __ __ ___ /,-._.--~' |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/fetchngs v1.12.0-g8ec2d93------------------------------------------------------Core Nextflow options revision : master runName : ecstatic_waddington containerEngine : singularity launchDir : /home/sibbe/Documents/projects/oomycete-gene-conservation/transcript-presence-absence-conservation workDir : /home/sibbe/Documents/projects/oomycete-gene-conservation/transcript-presence-absence-conservation/work projectDir : /home/sibbe/.nextflow/assets/nf-core/fetchngs userName : sibbe profile : singularity configFiles : Input/output options input : /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/ids/plasmopara_viticola_INRA-PV221.identifiers.csv nf_core_pipeline: rnaseq download_method : sratools outdir : /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/sequences/plasmopara_viticola_INRA-PV221!! Only displaying parameters that differ from the pipeline defaults !!------------------------------------------------------If you use nf-core/fetchngs for your analysis please cite:* The pipeline https://doi.org/10.5281/zenodo.5070524* The nf-core framework https://doi.org/10.1038/s41587-020-0439-x* Software dependencies https://github.com/nf-core/fetchngs/blob/master/CITATIONS.md------------------------------------------------------executor > local (2)[9e/7719ed] NFCORE_FETCHNGS:SRA:SRA_IDS_TO_RUNINFO (PRJNA121473) [100%] 1 of 1 ✔[a7/1bc8ca] NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP (1) [ 0%] 0 of 1executor > local (2)[9e/7719ed] NFCORE_FETCHNGS:SRA:SRA_IDS_TO_RUNINFO (PRJNA121473) [100%] 1 of 1 ✔[a7/1bc8ca] NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP (1) [ 0%] 0 of 1 ✘executor > local (2)[9e/7719ed] NFCORE_FETCHNGS:SRA:SRA_IDS_TO_RUNINFO (PRJNA121473) [100%] 1 of 1 ✔[a7/1bc8ca] NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP (1) [ 0%] 0 of 1 ✘[- ] NFCORE_FETCHNGS:SRA:SRA_FASTQ_FTP -[- ] NFC…TQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:CUSTOM_SRATOOLSNCBISETTINGS -[- ] NFC…GS:SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_PREFETCH -[- ] NFC…SRA:FASTQ_DOWNLOAD_PREFETCH_FASTERQDUMP_SRATOOLS:SRATOOLS_FASTERQDUMP -[- ] NFCORE_FETCHNGS:SRA:ASPERA_CLI -[- ] NFCORE_FETCHNGS:SRA:SRA_TO_SAMPLESHEET -[- ] NFCORE_FETCHNGS:SRA:MULTIQC_MAPPINGS_CONFIG -Execution cancelled -- Finishing pending tasks before exit-[nf-core/fetchngs] Pipeline completed with errors-WARN: ============================================================================= Please double-check the samplesheet that has been auto-created by the pipeline. Public databases don't reliably hold information such as strandedness information, controls etc All of the sample metadata obtained from the ENA has been appended as additional columns to help you manually curate the samplesheet before running nf-core/other pipelines.===================================================================================ERROR ~ Error executing process > 'NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP (1)'Caused by: Missing output file(s) `*.tsv` expected by process `NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP (1)` (note: input files are not included in the default matching set)Command executed: sra_runinfo_to_ftp.py \ PRJNA121473.runinfo.tsv \ PRJNA121473.runinfo_ftp.tsv cat <<-END_VERSIONS > versions.yml "NFCORE_FETCHNGS:SRA:SRA_RUNINFO_TO_FTP": python: $(python --version | sed 's/Python //g') END_VERSIONSCommand exit status: 0Command output: (empty)Command error: INFO: Converting SIF file to temporary sandbox... INFO: Cleaning up image...Work dir: /home/sibbe/Documents/projects/oomycete-gene-conservation/transcript-presence-absence-conservation/work/a7/1bc8caf2020933e67ea7937d4d1bdbContainer: /home/sibbe/Documents/projects/oomycete-gene-conservation/transcript-presence-absence-conservation/work/singularity/depot.galaxyproject.org-singularity-python-3.9--1.imgTip: when you have fixed the problem you can continue the execution adding the option `-resume` to the run command line -- Check '.nextflow.log' file for detailsRuleException:CalledProcessError in file /home/sibbe/Documents/projects/oomycete-gene-conservation/transcript-presence-absence-conservation/workflow/Snakefile, line 67:Command 'set -euo pipefail; nextflow run nf-core/fetchngs --input /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/ids/plasmopara_viticola_INRA-PV221.identifiers.csv --outdir /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/sequences/plasmopara_viticola_INRA-PV221 -profile singularity --nf_core_pipeline rnaseq --download_method sratools' returned non-zero exit status 1.[Mon May 12 14:15:14 2025]Error in rule download_sra: jobid: 3 input: /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/ids/plasmopara_viticola_INRA-PV221.identifiers.csv output: /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/sequences/plasmopara_viticola_INRA-PV221 shell: nextflow run nf-core/fetchngs --input /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/ids/plasmopara_viticola_INRA-PV221.identifiers.csv --outdir /net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/sequences/plasmopara_viticola_INRA-PV221 -profile singularity --nf_core_pipeline rnaseq --download_method sratools (one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)Removing output files of failed job download_sra since they might be corrupted:/net/chaperone/linuxhome/michael-group/sibbe/results/rna-presence-absence/sequences/plasmopara_viticola_INRA-PV221Shutting down, this might take some time.Exiting because a job execution failed. Look above for error messageComplete log: .snakemake/log/2025-05-12T141503.370842.snakemake.logWorkflowError:At least one job did not complete successfully.
Relevant files
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The text was updated successfully, but these errors were encountered:
Description of the bug
I am trying to download the transcriptomic data for PRJNA121473. From the error message I do not understand what I can change to fix my issue.
Command used and terminal output
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: