|
1 | 1 | # Explore demultiplexing, the experimental method
|
| 2 | + |
| 3 | +```{admonition} Note |
| 4 | + :kind: warning |
| 5 | + This mode of anglerfish work-in-progress and as such is not feature complete and might give sub-optimal results |
| 6 | +``` |
| 7 | + |
| 8 | +This is a way of running anglerfish without using a samplesheet. The functionality is however quite different from `anglerfish run` |
| 9 | +It will attempt, using read alignment, to find which adapter types are present in a given pool and their index length(s). |
| 10 | + |
| 11 | +The basic usage of `anglerfish explore` expects two arguments, one for a single input fastq file `-f` and one for the output directory `-o`. Example shown for a pool where it finds `truseq_dual` adapters with 8+8 bp index length. |
| 12 | + |
| 13 | +``` |
| 14 | +anglerfish explore -f fastq_passed/concat.fastq.gz -o explore |
| 15 | +INFO:explore:Running anglerfish explore |
| 16 | +INFO:explore:Run uuid 6b1c9b21-029f-4fc3-b28a-9214c776048a |
| 17 | +INFO:explore:Aligning illumina_ud |
| 18 | +INFO:explore:Aligning truseq |
| 19 | +INFO:explore:Aligning truseq_dual |
| 20 | +INFO:explore:Aligning truseq_umi |
| 21 | +INFO:explore:Aligning nextera_legacy |
| 22 | +INFO:explore:Aligning nextera_dual |
| 23 | +INFO:explore:Parsing illumina_ud |
| 24 | +INFO:explore:illumina_ud:i5 had 0 good hits. |
| 25 | +INFO:explore:illumina_ud:i7 had 0 good hits. |
| 26 | +INFO:explore:Adaptor illumina_ud is excluded from the analysis |
| 27 | +INFO:explore:Parsing truseq |
| 28 | +INFO:explore:truseq:i5 had 0 good hits. |
| 29 | +INFO:explore:truseq:i7 had 5810 good hits. |
| 30 | +INFO:explore:Adaptor truseq is excluded from the analysis |
| 31 | +INFO:explore:Parsing truseq_dual |
| 32 | +INFO:explore:truseq_dual:i5 had 779 good hits. |
| 33 | +INFO:explore:truseq_dual:i7 had 5810 good hits. |
| 34 | +INFO:explore:Adaptor truseq_dual is included in the analysis |
| 35 | +INFO:explore:Parsing truseq_umi |
| 36 | +INFO:explore:truseq_umi:i5 had 779 good hits. |
| 37 | +INFO:explore:truseq_umi:i7 had 0 good hits. |
| 38 | +INFO:explore:Adaptor truseq_umi is excluded from the analysis |
| 39 | +INFO:explore:Parsing nextera_legacy |
| 40 | +INFO:explore:nextera_legacy:i5 had 0 good hits. |
| 41 | +INFO:explore:nextera_legacy:i7 had 0 good hits. |
| 42 | +INFO:explore:Adaptor nextera_legacy is excluded from the analysis |
| 43 | +INFO:explore:Parsing nextera_dual |
| 44 | +INFO:explore:nextera_dual:i5 had 0 good hits. |
| 45 | +INFO:explore:nextera_dual:i7 had 0 good hits. |
| 46 | +INFO:explore:Adaptor nextera_dual is excluded from the analysis |
| 47 | +INFO:explore:truseq_dual:i5 had 779 good hits with median insert length 8.0 |
| 48 | +INFO:explore:truseq_dual:i5 insert length histogram saved explore/truseq_dual_i5.hist.csv |
| 49 | +INFO:explore:truseq_dual:i7 had 5810 good hits with median insert length 8.0 |
| 50 | +INFO:explore:truseq_dual:i7 insert length histogram saved explore/truseq_dual_i7.hist.csv |
| 51 | +``` |
0 commit comments