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Add a WIP notice for explore
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README.md

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@@ -11,7 +11,7 @@ Anglerfish is a tool designed to demultiplex Illumina libraries sequenced on Oxf
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flowcells. The primary purpose for this would be to do QC, i.e. to check pool balancing, assess
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contamination, library insert sizes and so on.
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For more information on how this can be used, please see this [poster](docs/AGBT_poster_20200214.pdf).
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For more information please read the [documentation](https://anglerfish-dev.readthedocs.io/en/latest/index.html) or see this [poster](docs/AGBT_poster_20200214.pdf).
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## Installation
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docs/anglerfish-explore.md

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# Explore demultiplexing, the experimental method
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```{admonition} Note
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:kind: warning
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This mode of anglerfish work-in-progress and as such is not feature complete and might give sub-optimal results
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```
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This is a way of running anglerfish without using a samplesheet. The functionality is however quite different from `anglerfish run`
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It will attempt, using read alignment, to find which adapter types are present in a given pool and their index length(s).
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The basic usage of `anglerfish explore` expects two arguments, one for a single input fastq file `-f` and one for the output directory `-o`. Example shown for a pool where it finds `truseq_dual` adapters with 8+8 bp index length.
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```
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anglerfish explore -f fastq_passed/concat.fastq.gz -o explore
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INFO:explore:Running anglerfish explore
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INFO:explore:Run uuid 6b1c9b21-029f-4fc3-b28a-9214c776048a
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INFO:explore:Aligning illumina_ud
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INFO:explore:Aligning truseq
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INFO:explore:Aligning truseq_dual
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INFO:explore:Aligning truseq_umi
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INFO:explore:Aligning nextera_legacy
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INFO:explore:Aligning nextera_dual
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INFO:explore:Parsing illumina_ud
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INFO:explore:illumina_ud:i5 had 0 good hits.
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INFO:explore:illumina_ud:i7 had 0 good hits.
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INFO:explore:Adaptor illumina_ud is excluded from the analysis
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INFO:explore:Parsing truseq
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INFO:explore:truseq:i5 had 0 good hits.
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INFO:explore:truseq:i7 had 5810 good hits.
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INFO:explore:Adaptor truseq is excluded from the analysis
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INFO:explore:Parsing truseq_dual
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INFO:explore:truseq_dual:i5 had 779 good hits.
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INFO:explore:truseq_dual:i7 had 5810 good hits.
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INFO:explore:Adaptor truseq_dual is included in the analysis
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INFO:explore:Parsing truseq_umi
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INFO:explore:truseq_umi:i5 had 779 good hits.
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INFO:explore:truseq_umi:i7 had 0 good hits.
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INFO:explore:Adaptor truseq_umi is excluded from the analysis
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INFO:explore:Parsing nextera_legacy
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INFO:explore:nextera_legacy:i5 had 0 good hits.
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INFO:explore:nextera_legacy:i7 had 0 good hits.
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INFO:explore:Adaptor nextera_legacy is excluded from the analysis
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INFO:explore:Parsing nextera_dual
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INFO:explore:nextera_dual:i5 had 0 good hits.
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INFO:explore:nextera_dual:i7 had 0 good hits.
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INFO:explore:Adaptor nextera_dual is excluded from the analysis
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INFO:explore:truseq_dual:i5 had 779 good hits with median insert length 8.0
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INFO:explore:truseq_dual:i5 insert length histogram saved explore/truseq_dual_i5.hist.csv
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INFO:explore:truseq_dual:i7 had 5810 good hits with median insert length 8.0
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INFO:explore:truseq_dual:i7 insert length histogram saved explore/truseq_dual_i7.hist.csv
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```

docs/index.rst

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anglerfish
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anglerfish-run
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anglerfish-explore
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troubleshooting

docs/troubleshooting.md

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