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With multiple potential sources of TCR count tables, the pipeline should be able to handle inputs beyond Adaptive. Particularly relevant is taking outputs from CellRanger for scRNA TCR sequencing, to enable pseudobulking of single-cell data (see #33).
Bare minimum columns:
full nucleotide sequence
full nucleotide aa sequence (can infer from full nuc seq)
CDR3 nucleotide (can infer from full nuc seq)
CDR3 aa sequence (can infer from full nuc seq)
count
VJ identifications
We will need to agree on a standard to conform Adaptive and any other imported count tables to; AIRR is a possible standard widely-adopted by the open source community. Other pipelines have been certified as AIRR-compliant, and I will investigate how we can conform our inputs to this standard.
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With multiple potential sources of TCR count tables, the pipeline should be able to handle inputs beyond Adaptive. Particularly relevant is taking outputs from CellRanger for scRNA TCR sequencing, to enable pseudobulking of single-cell data (see #33).
Bare minimum columns:
We will need to agree on a standard to conform Adaptive and any other imported count tables to; AIRR is a possible standard widely-adopted by the open source community. Other pipelines have been certified as AIRR-compliant, and I will investigate how we can conform our inputs to this standard.
The text was updated successfully, but these errors were encountered: